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Vimentin and keratin intermediate filament systems in cultured PtK2 epithelial cells are interrelated.
Author(s) -
Klymkowsky M.W.
Publication year - 1982
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1982.tb01141.x
Subject(s) - vimentin , intermediate filament , keratin , protein filament , biology , keratin 8 , intermediate filament protein , cytoskeleton , keratin 6a , microbiology and biotechnology , cell , biochemistry , immunology , immunohistochemistry , genetics
Certain cultured epithelial cells contain separate vimentin and keratin‐type intermediate filament networks. The intracellular injection of monoclonal antibodies directed against either vimentin or keratin filaments into PtK2 cultured epithelial cells specifically disrupted the organization of both filament types. Neither antibody had any effect when injected into cells which, while containing vimentin or keratin filaments, lacked the specific filament type which that antibody recognized. These experiments suggest that keratin and vimentin filament networks are associated in some way with one another.