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Regulation of heat‐shock genes: a DNA sequence upstream of Drosophila hsp70 genes is essential for their induction in monkey cells.
Author(s) -
Mirault M.E.,
Southgate R.,
Delwart E.
Publication year - 1982
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1982.tb00025.x
Subject(s) - biology , drosophila melanogaster , gene , hsp70 , schneider 2 cells , microbiology and biotechnology , heat shock protein , transcription (linguistics) , rna , genetics , rna interference , linguistics , philosophy
Heat‐shock genes coding for heat‐shock protein 70 (HSP70) in Drosophila melanogaster were subcloned into an SV40/plasmid recombinant capable of replication in permissive monkey COS cells. Following transfection of COS cells, no significant amount of Drosophila hsp70 RNA was detected at 37 degrees C. In contrast, a heat‐shock at 43 degrees C or arsenite poisoning at 37 degrees C induced the massive production of Drosophila hsp70 RNA of correct size and faithful 5′ ends. After heat‐shock, the efficiency of hsp70 transcription in COS cells containing 2‐4 X 10(4) gene copies was found to be 15‐30% of that measured in Drosophila, on a per gene basis. By testing a series of 5′ deletion mutants in this inducible transcription assay it was found that a sequence less than 70 bp long, directly upstream of the hsp70 gene, was essential for the heat or arsenite induction of transcription.