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Expression of hepatitis B surface antigen in unselected cell culture transfected with recircularized HBV DNA.
Author(s) -
Wang Y.,
SchäferRidder M.,
Stratowa C.,
Wong T.K.,
Hofschneider P.H.
Publication year - 1982
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1982.tb00015.x
Subject(s) - hbsag , biology , hepatitis b virus , microbiology and biotechnology , transfection , antigen , virology , recombinant dna , cell culture , gene , immunology , virus , genetics
Hepatitis B virus (HBV) DNA was isolated from the recombinant plasmid pA01 ‐HBV and recircularized . Immediately after introduction of this DNA into mouse fibroblasts (NIH 3T3) we observed increasing release of hepatitis B surface antigen (HBsAg) into the culture medium. Later production of HBsAg declined to a lower but constant level. No dominant selective marker and foreign promoter were necessary in this system, which therefore can be used for the study of regulation of HBsAg expression.