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Transient expression of the chicken lysozyme gene after transfer into human cells.
Author(s) -
Matthias P.D.,
Renkawitz R.,
Grez M.,
Schütz G.
Publication year - 1982
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1982.tb00014.x
Subject(s) - biology , lysozyme , gene transfer , gene expression , gene , transient (computer programming) , microbiology and biotechnology , genetics , computer science , operating system
The chicken lysozyme gene was inserted into an SV40‐based plasmid vector, and the recombinants were transfected into the human cell lines HeLa and MCF‐7. Correct and efficient transient expression directed by the lysozyme promoter was found in both of these cell lines, as determined by S1 nuclease mapping and Northern blot analysis of the RNAs made. SV40 sequences dramatically enhance the expression of the lysozyme gene. This enhancing effect is only acting in cis and is distance/orientation dependent, since clones containing the lysozyme gene in either orientation produce different amounts of correct lysozyme transcripts. The transfected lysozyme gene was not induced by steroid hormone treatment of the cells.