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Visualization of Z sequences in form V of pBR322 by immuno‐electron microscopy.
Author(s) -
Lang M.C.,
Malfoy B.,
Freund A.M.,
Daune M.,
Leng M.
Publication year - 1982
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1982.tb00005.x
Subject(s) - pbr322 , dna , biology , microbiology and biotechnology , genetics , plasmid
Form V DNA has been prepared from pBR322 DNA by annealing covalently closed complementary single strands. Specific rabbit antibodies to Z‐DNA were shown by radioimmunoassay and electron microscopy to react with form V DNA of pBR322. The bound antibodies were visualized either directly (on synthetic polynucleotides in Z‐form), or after reaction with goat anti‐rabbit immunoglobulin labeled with ferritin (on form V DNA).