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Fiber Optic Fluorescence Microprobe for Endodontic Diagnosis
Author(s) -
Sarkissian Ani,
Le Ashley Nga
Publication year - 2005
Publication title -
journal of dental education
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 68
eISSN - 1930-7837
pISSN - 0022-0337
DOI - 10.1002/j.0022-0337.2005.69.6.tb03946.x
Subject(s) - dentin , root canal , pulp (tooth) , microprobe , dentinal tubule , fluorescence , endodontic therapy , dentistry , autofluorescence , odontoblast , materials science , chemistry , pathology , biomedical engineering , medicine , optics , mineralogy , physics
Successful endodontic therapy requires total debridement as well as complete obturation of the root canal to the cemento‐dentinal junction. The goal of this study was to investigate the feasibility of using quantitative fluorescence spectroscopy for the detection and localization of pathological dentin, pulpal remnants, and microorganisms within the root canal. Specific aims were to identify: 1) characteristic excitation/emission spectra for healthy dentin, decayed dentin, enamel, and pulp; 2) the potential of specific spectral data for differentiating between these tissues; and 3) the potential of spectral data for detecting the presence and identifying four common endodontic pathogens. Fluorescence spectra were determined in the tissues of permanent human teeth, extirpated healthy and necrotic pulps, and four endodontic pathogens. Excitation/emission spectra were collected at 366nm, 405nm, and 440nm excitation. Marked differences in spectral signatures between the different tissues under investigation were observed. We postulate that the differences in fluorescence spectra of decayed vs. healthy dentin are due to the loss of mineralized tissue components and increased organic presence and water in these tissues. Pulpal tissue showed distinctly different fluorescence spectra from healthy and decayed dentin, providing a basis for differentiating between tissue categories. Each bacterial species demonstrated distinct spectral emission patterns.