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Molecular organization of the gene encoding Xenopus Laevis transforming growth factor‐β5
Author(s) -
Vempati Uma Devi,
Kondaiah Paturu
Publication year - 1998
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1002/iub.7510450517
Subject(s) - xenopus , exon , intron , gene isoform , transforming growth factor , gene , biology , complementary dna , alternative splicing , genetics , microbiology and biotechnology , rna splicing , rna
Transforming growth factor‐βs (TGF‐βs) are multifunctional polypeptides, known to influence proliferation and differentiation of many cell types. TGF‐β5 cDNA was cloned from Xenopus laevis and this isoform is unique to the amphibians. Here, we report the isolation and characterization of the TGF‐β5 genomic clones to determine the structure of TGF‐β5 gene. The gene consists of seven exons and all intron‐exon boundaries follow the GT‐AG consensus. The organization of TGF‐β5 gene was identical to that of the mammalian TGF‐β5 isoforms, with the exception of position of the first splice junction. We determined the size of TGF‐β5 gene to be approximately 20 kb.