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Rapd profile variation amongst provenances of neem
Author(s) -
Farooqui Nuzhat,
Ranade S.A.,
Sane P.V.
Publication year - 1998
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1002/iub.7510450511
Subject(s) - rapd , biology , genetic variation , variation (astronomy) , botany , genetics , genetic diversity , demography , sociology , gene , population , physics , astrophysics
Neem, described as a tree for solving global problems, is an evergreen, long‐lived, multipurpose tree of the tropics with a wide distribution range in India. It is believed to be highly cross‐pollinated. Inter‐provenance variations have been reported in neem in case of morphological and physiological characters. Yet no reports about the genetic determinism for these variations are available to our knowledge. In order to have an idea about the extent and/or nature of genetic (DNA) variation in neem, the powerful RAPD technique has been employed. RAPD profiles of 34 accessions/provenances of neem were generated with 200 decamer random primers, of which the data from the 49 primers, that resulted in reproducible amplification products, were considered for analysis. Based on the presence/absence of bands, a similarity matrix was computed. Dendrogram was constructed by UPGMA method based on the pairwise similarities amongst the RAPD profiles. The similarities in RAPD profiles amongst the different DNAs was more than that expected due to the cross‐pollinated nature of the tree and furthermore, these more‐than‐expected similarities were not due to random chance. These results suggest that neem may have a narrow genetic base.