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Changes in the two‐dimensional electrophoresis pattern of the Parkinson's disease related protein DJ‐1 in human SH‐SY5Y neuroblastoma cells after dopamine treatment
Author(s) -
Alberio Tiziana,
Colapinto Monica,
Natale Massimo,
Ravizza Raffaella,
Gariboldi Marzia B.,
Bucci Enrico M.,
Lopiano Leonardo,
Fasano Mauro
Publication year - 2010
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1002/iub.371
Subject(s) - dopamine , sh sy5y , dopaminergic , parkinson's disease , oxidative stress , catecholaminergic , neuroblastoma , oxidative phosphorylation , microbiology and biotechnology , chemistry , biology , biochemistry , endocrinology , medicine , disease , cell culture , genetics
DJ‐1 is a mitochondrial protein linked to Parkinson's disease. DJ‐1 has been suggested to have several possible functions, although it has been mainly associated to oxidative stress defence. Changes in the two‐dimensional electrophoresis pattern have been thoroughly described as a consequence of oxidative modification of the Cys106 residue. There is accumulating evidence supporting a specific role of DJ‐1 in protecting dopaminergic neurons from dopamine itself. By exposing SH‐SY5Y human neuroblastoma catecholaminergic cells to dopamine, we observed a specific increase in the most acidic forms in the DJ‐1 two‐dimensional electrophoresis pattern together with a significant decrease of the most basic spot. Unlike cells exposed to generic oxidative conditions, no additional shift was observed. The results are corroborated by a meta‐analysis of the literature showing that in the absence of dopamine treatment the specific acidic form is underrepresented. © 2010 IUBMB IUBMB Life, 62(9): 688–692, 2010