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The long and winding road to RNA editing in plant mitochondria: The Tübingen‐Berlin chapter
Author(s) -
Brennicke Axel
Publication year - 2009
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1002/iub.269
Subject(s) - rna editing , mitochondrial dna , complementary dna , biology , rna , cloning (programming) , genetics , sequence (biology) , mitochondrion , computational biology , gene , computer science , programming language
It took several independent observations of C‐to‐T differences between genomic mtDNA sequences and corresponding complementary DNA (cDNA) sequences before RNA editing in plant mitochondria was accepted as a fact by the group at Tübingen and later Berlin (Hiesel et al., Science 246 (1989) 1632–1634). The first such deviating sequence runs were critically viewed in the lab as being errors of some kind, most likely cloning artifacts, which occur only too frequently. Several such cDNA‐mtDNA differences identified in independent cDNA clones in different libraries and finally CGG to TGG codon changes dispelled the skeptical view, and this phenomenon was finally recognized as plant mitochondrial RNA editing of a type similar to the apolipoprotein B RNA editing in mammals. © 2009 IUBMB IUBMB Life, 61: 1105–1109, 2009.