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Circ_0109046 promotes the malignancy of endometrial carcinoma cells through the microRNA ‐105/ SOX9 /Wnt/β‐catenin axis
Author(s) -
Li Yanyan,
Liu Jinyu,
Piao Jinxia,
Ou Jian,
Zhu Xiaoyan
Publication year - 2021
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1002/iub.2415
Subject(s) - downregulation and upregulation , wnt signaling pathway , gene silencing , microrna , cancer research , catenin , malignancy , apoptosis , sox9 , chemistry , cell growth , biology , microbiology and biotechnology , medicine , signal transduction , gene expression , gene , biochemistry
Emerging evidence suggests the important involvements of circular RNAs (circRNAs) in cancer progression. This study focuses on the function of Circ_0109046 on the malignancy of endometrial carcinoma (EC) cells and the molecules involved. First, high expression of Circ_0109046 was found in EC tissues compared to the adjacent tissues, and it predicted unfavorable prognosis in patients. Similarly, high expression of Circ_0109046 was confirmed in EC cells relative to that in normal endometrial epithelial cells. Silencing of Circ_0109046 in AN3‐CA cells inhibited proliferation and aggressiveness but increased apoptosis of cells. Circ_0109046 was mainly sub‐localized in cytoplasm, and it mediated SOX9 expression through sponging microRNA (miR)‐105. The proliferation and aggressiveness of EC cells suppressed by Circ_0109046 downregulation was recovered upon SOX9 overexpression. SOX9 activated the Wnt/β‐catenin pathway. Furthermore, downregulation of Circ_0109046 reduced the growth of xenograft tumors in nude mice. This study evidenced that Circ_0109046 upregulates SOX9 expression through sponging miR105, leading to activation of Wnt/β‐catenin signaling and the malignant growth of EC. This study may offer novel understanding in EC treatment.