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The prospective curative role of lipoxin A 4 in induced gastric ulcer in rats: Possible involvement of mitochondrial dynamics signaling pathway
Author(s) -
Madi Nermin M.,
Ibrahim Rowida R.,
Alghazaly Ghada M.,
Marea Karima E.,
ElSaka Mervat H.
Publication year - 2020
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1002/iub.2260
Subject(s) - immunohistochemistry , gastric mucosa , apoptosis , omeprazole , mitochondrion , ulcer index , medicine , gastroenterology , reactive oxygen species , stomach , pathology , pharmacology , chemistry , endocrinology , biochemistry
This study purposed to examine the prospective curative role of lipoxin A 4 (LXA 4 ) in induced gastric ulcer in rats and explore the possible involvement of mitochondrial dynamics signaling pathway. Forty‐eight male Wistar rats were divided into four groups: control, indomethacin (IND), IND + omeprazole (IND + Omez), and IND+ LXA 4 groups. At the end of the experiment, the gastric pH, gastric fluid volume, total gastric acidity, ulcer index, and curative index were estimated. The gene expression of mitochondrial related protein 1 and mitofusin 2 were determined. In addition, some mitochondrial parameters include mitochondrial transmembrane potential, complex‐I activity and reactive oxygen species were measured. Also, some gastric biochemical parameters, histopathological, and immunohistochemical analyses of the gastric mucosa were determined. We found that IND induced gastric ulcer, as manifested by the biochemical, histopathological, and immunohistochemical analyses. Both Omez and LXA 4 treatment for 15 days alleviated the IND‐induced gastric ulcer as explored by ameliorating the biochemical, histopathological, and immunohistochemical findings. We concluded that LXA 4 mitigated the IND‐induced gastric ulcer via improving the mitochondrial dynamic imbalance and mitochondrial dysfunction, in addition to its anti‐apoptotic, anti‐inflammatory, and antioxidant properties.