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Downregulated CHI3L1 alleviates skeletal muscle stem cell injury in a mouse model of sepsis
Author(s) -
Li Fuxing,
Sheng Zhiyong,
Lan Haibing,
Xu Jianning,
Li Juxiang
Publication year - 2020
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1002/iub.2156
Subject(s) - sepsis , skeletal muscle , stem cell , gene silencing , biology , microbiology and biotechnology , downregulation and upregulation , lipopolysaccharide , inflammation , cancer research , immunology , endocrinology , gene , biochemistry
Sepsis is an acute systemic inflammatory response of the body to microbial infection and a life‐threatening condition associated with multiple organ failure. Recent data suggest that sepsis survivors present with long‐term myopathy due to the dysfunction of skeletal muscle stem cells and satellite cells. Accumulating studies have implicated chitinase‐3‐like‐1 protein (CHI3L1) in a variety of infectious diseases, specifically sepsis. Therefore, the aim of the present study is to elucidate the potential mechanism by which CHI3L1 is involved in the injury of skeletal muscle stem cells in mouse models of sepsis. An in vitro cell model was developed by lipopolysaccharide (LPS) and in vivo mouse model of sepsis was induced by CRP‐like protein (CLP). To elucidate the biological significance behind the silencing of CHI3L1, modeled skeletal muscle stem cells and mice were treated with siRNA against CHI3L1 or overexpressed CHI3L1. Highly expressed CHI3L1 was found in skeletal muscle tissues of mice with sepsis. Besides, siRNA‐mediated silencing of CHI3L1 was revealed to increase Bcl‐2 expression along with cell proliferation, while diminishing Bax expression, cell apopstosis as well as serum levels of TNF‐α, IL‐1β, INF‐γ, IL‐10, and IL‐6. Taken conjointly, this present study provided evidence suggesting that downregulation of CHI3L1 has the potential to prevent the injury of skeletal muscle stem cells in mice with sepsis. Collectively, CHI3L1 may serve as a valuable therapeutic strategy in alleviating sepsis.