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Phytol as an anticarcinogenic and antitumoral agent: An in vivo study in swiss mice with DMBA‐Induced breast cancer
Author(s) -
de Alencar Marcus Vinícius Oliveira Barros,
Islam Muhammad Torequl,
de Lima Rosália Maria Torres,
Paz Márcia Fernanda Correia Jardim,
Reis Antonielly Campinho,
Mata Ana Maria Oliveira Ferreira,
Filho José Williams Gomes de Oliveira,
Cerqueira Gilberto Santos,
Ferreira Paulo Michel Pinheiro,
e Sousa João Marcelo de Castro,
Mubarak Mohammad S.,
MeloCavalcante Ana Amélia de Carvalho
Publication year - 2019
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1002/iub.1952
Subject(s) - genotoxicity , cancer , carcinogen , dmba , comet assay , pharmacology , in vivo , cancer research , toxicity , breast cancer , histopathology , cyclophosphamide , medicine , carcinogenesis , pathology , biology , dna damage , chemotherapy , biochemistry , dna , microbiology and biotechnology
Phytol (PHY) (3,7,11,15‐tetramethylhexadec‐2‐en‐1‐ol) exhibits various pharmacological properties including toxicity and cytotoxicity, and exerts antitumor activity. Owing to the urgent need of new pharmaceutical formulations for breast cancer therapy, this study aimed at the evaluation of antitumor activity of PHY in 7,12‐dimethylbenzanthracene‐cancer‐induced animal model. Comet assay was employed to evaluate the cytogenetics, DNA repair, and antigenotoxic activities of PHY in neoplastic (breast) and non‐neoplastic rodent cells (bone marrow, lymphocytes, and liver). Additionally, hematological, biochemical, histopathological, and immunohistochemical analyses were carried out in experimental animals. Thirty nonpregnant female mice ( n = 5) underwent 7 weeks treatment with 6 mg/kg pro‐carcinogen, PHY (4 mg/kg), and cyclophosphamide (25 mg/kg). Induction of cancer was confirmed by histopathology and immunohistochemistry for Ki‐67. Results suggest that PHY exhibits low toxicity in comparison with other groups in hematological, biochemical, histopathological, and organ size parameters. Additionally, PHY showed modulatory effects on the pro‐carcinogen, and induced genotoxicity and apoptosis in breast cancer cells. Furthermore, it showed a DNA damage repair capacity in mouse lymphocytes. These data indicate that PHY may have the potential as an anticancer candidate in pharmaceutical consumption. © 2018 IUBMB Life, 71(1):200–212, 2019