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RecG wed : A probable novel regulator in the resolution of branched DNA structures in mycobacteria
Author(s) -
Singh Amandeep,
Vijayan M.,
Nagaraju Ganesh
Publication year - 2018
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1002/iub.1881
Subject(s) - mycobacterium smegmatis , dna , helicase , biology , genetics , homologous recombination , genome , computational biology , dna repair , gene , chemistry , mycobacterium tuberculosis , tuberculosis , rna , pathology , medicine
Structure‐specific helicases, such as RecG, play an important role in the resolution of recombination intermediates. A bioinformatic analysis of mycobacterial genomes led to the identification of a protein (RecG wed ) with a C‐terminal “edge” domain, similar to the wedge domain of RecG. RecG wed is predominately found in the phylum Actinobacteria and in few human pathogens. Mycobacterium smegmatis RecG wed was able to bind branched DNA structures in vitro but failed to interact with single‐ or double‐stranded DNA. The expression of recG wed in M. smegmatis cells was up‐regulated during stationary phase/UV damage and down‐regulated during MMS/H 2 O 2 treatment. These observations indicate the possible involvement of RecG wed in transactions during recombination events, that proceed though branched DNA intermediates. © 2018 IUBMB Life, 70(8):786–794, 2018