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Impaired antioxidant enzyme functions with increased lipid peroxidation in epithelial ovarian cancer
Author(s) -
Caglayan Aydan,
Katlan Doruk Cevdi,
Selçuk Tuncer Zafer,
Yüce Kunter,
Sayal Hasan Berkan,
Coşkun Salman Mehmet,
KocerGumusel Belma
Publication year - 2017
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1002/iub.1675
Subject(s) - superoxide dismutase , lipid peroxidation , glutathione peroxidase , chemistry , gpx1 , malondialdehyde , catalase , medicine , endocrinology , oxidative stress , antioxidant , ovarian cancer , ovarian tumor , cancer , biochemistry
Abstract We aimed to identify the possible role of oxidant–antioxidant status in epithelial ovarian cancer (EOC) by measuring (a) antioxidant enzyme (AOE) activities [total superoxide dismutase (SOD total ), manganese‐SOD (Mn‐SOD), copper,zinc‐SOD (Cu,Zn‐SOD), catalase (CAT) and glutathione peroxidase (GPx1)], (b) Mn‐SOD protein expression, (c) lipid peroxidation markers [malondialdehyde (MDA), 8‐epi‐prostaglandin‐F2α (8‐epi‐PGF2α)] and by evaluating the possible correlations between tumor biomarkers, reproductive hormone levels and all measured parameters, comprehensively. The data obtained from the patients with EOC (M, n = 26) evaluated according to the histopathological/clinical characteristics of tumors and compared with data of healthy controls [C tissue (C1) and C blood/urine (C2), n = 30, respectively). Significantly, low activities of tumor SOD total (52%), Mn‐SOD (42%), Cu,Zn‐SOD (55%); high activities of tumor and erythrocyte CAT (66%, 33% respectively) and tumor GPx1 (60%); high levels of tumor Mn‐SOD protein expression; tumor MDA (193%) and urinary 8‐epi‐PGF2α (179%) were observed in serous EOC tumors (M1, n = 18) compared with controls ( P < 0.05). However, higher levels of tumor MDA, Mn‐SOD protein expression and urinary 8‐epi‐PGF2α were observed along with lower tumor CAT activity in poorly differentiated or undifferentiated (grade 3, G 3) versus well or moderately well differentiated (grade 1‐2, G 1‐2) serous EOC tumors. Obtained data indicate the presence of a severe redox imbalance in EOC and draw attention to the criticial role of AOEs in the pathogenesis of the disease. © 2017 IUBMB Life, 69(10):802–813, 2017