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S ‐nitrosomycothiol reductase and mycothiol are required for survival under aldehyde stress and biofilm formation in Mycobacterium smegmatis
Author(s) -
Vargas Derek,
Hageman Samantha,
Gulati Megha,
Nobile Clarissa J.,
Rawat Mamta
Publication year - 2016
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1002/iub.1524
Subject(s) - mycobacterium smegmatis , biofilm , chemistry , microbiology and biotechnology , biology , mycobacterium tuberculosis , medicine , bacteria , genetics , tuberculosis , pathology
We show that Mycobacterium smegmatis mutants disrupted in mscR , coding for a dual function S ‐nitrosomycothiol reductase and formaldehyde dehydrogenase, and mshC , coding for a mycothiol ligase and lacking mycothiol (MSH), are more susceptible to S ‐nitrosoglutathione (GSNO) and aldehydes than wild type. MSH is a cofactor for MscR, and both mshC and mscR are induced by GSNO and aldehydes. We also show that a mutant disrupted in egtA , coding for a γ‐glutamyl cysteine synthetase and lacking in ergothioneine, is sensitive to nitrosative stress but not to aldehydes. In addition, we find that MSH and S ‐nitrosomycothiol reductase are required for normal biofilm formation in M. smegmatis , suggesting potential new therapeutic pathways to target to inhibit or disrupt biofilm formation. © 2016 IUBMB Life, 68(8):621–628, 2016