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siRNA‐targeted COL8A1 inhibits proliferation, reduces invasion and enhances sensitivity to D ‐limonence treatment in hepatocarcinoma cells
Author(s) -
Zhao Yongfu,
Jia Li,
Mao Xiaoyan,
Xu Henggui,
Wang Bo,
Liu Yongji
Publication year - 2009
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1002/iub.151
Subject(s) - rna interference , small interfering rna , downregulation and upregulation , in vitro , rna , microbiology and biotechnology , cell culture , cell growth , cancer research , gene expression , chemistry , transfection , biology , gene , biochemistry , genetics
The COL8A1 (collagen type VIII, alpha‐1) gene, which encodes the alpha 1 chain of collagen, type VIII, may modulate migration, proliferation and adherence of various cells. Only very sparse information exists on COL8A1 expression in hepatocarcinoma. To investigate the possible role of COL8A1 in the mouse hepatocarcinoma cell line Hca‐F with highly metastatic potential in the lymph nodes, we used an RNA interference (RNAi) approach to silence COL8A1 expression. The results showed that a small interfering RNA (siRNA) targeted against COL8A1 significantly impeded Hca‐F cells proliferation and colony formation in soft agar. This reduction of COL8A1 expression also led to the decreased invasion of Hca‐F cells dramatically in vitro . Furthermore, the downregulation of COL8A1 expression also sensitized cells to the action of D ‐limonene. These data together provide insights into the function of COL8A1 and suggest that COL8A1 might represent a new potential target for gene therapy in hepatocarcinoma. © 2008 IUBMB IUBMB Life, 61(1): 74–79, 2009