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Coprisin exerts antibacterial effects by inducing apoptosis‐like death in E scherichia coli
Author(s) -
Choi Hyemin,
Hwang JaeSam,
Lee Dong Gun
Publication year - 2016
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1002/iub.1463
Subject(s) - apoptosis , dna fragmentation , annexin , phosphatidylserine , escherichia coli , programmed cell death , fragmentation (computing) , microbiology and biotechnology , blot , chemistry , dna damage , in situ nick end labeling , depolarization , terminal deoxynucleotidyl transferase , tunel assay , biology , dna , biochemistry , membrane , biophysics , phospholipid , ecology , gene
Apoptosis commonly occurs in eukaryotes to eliminate unwanted cells. In this study, we demonstrated that coprisin‐treated bacteria undergo cell death that is mechanistically and morphologically similar to apoptosis in eukaryotes. Time‐kill kinetic assay against Escherichia coli indicated that coprisin exerted bactericidal activity. The bactericidal mechanism was studied by terminal deoxynucleotidyl transferase dUTP nick end labeling analysis, followed by Western blotting. We confirmed coprisin‐induced DNA fragmentation and activation of the RecA protein as a SOS response. Furthermore, FITC‐VAD‐FMK, FITC‐Annexin V, and bis‐(1,3‐dibutylbarbituric acid) trimethineoxonol [DiBAC 4 (3)] staining showed that caspase‐like protein(s), such as RecA, were activated, and membrane alterations such as phosphatidylserine externalization and cytoplasmic depolarization were induced. Finally, 3′‐( p ‐hydroxyphenyl) fluorescein assay indicated that depolarization of membrane potential leads to hydroxyl radicals ( • OH) formation. Based on these results, we conclude that coprisin exerts bactericidal activity against E. coli by causing severe DNA damage, which induces apoptosis‐like death. © 2015 IUBMB Life, 68(1):72–78, 2016