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Pre‐existing interleukin 10 in cerebral arteries attenuates subsequent brain injury caused by ischemia/reperfusion
Author(s) -
Liang QiuJuan,
Jiang Mei,
Wang XinHong,
Le LiLi,
Xiang Meng,
Sun Ning,
Meng Dan,
Chen SiFeng
Publication year - 2015
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1002/iub.1429
Subject(s) - medicine , ischemia , stroke (engine) , middle cerebral artery , reperfusion injury , genetic enhancement , in situ hybridization , cerebral infarction , brain ischemia , infarction , anesthesia , pharmacology , pathology , gene expression , gene , biology , myocardial infarction , mechanical engineering , biochemistry , engineering
Recurrent stroke is difficult to treat and life threatening. Transfer of anti‐inflammatory gene is a potential gene therapy strategy for ischemic stroke. Using recombinant adeno‐associated viral vector 1 (rAAV1)‐mediated interleukin 10 (IL‐10), we investigated whether transfer of beneficial gene into the rat cerebral vessels during interventional treatment for initial stroke could attenuate brain injury caused by recurrent stroke. Male Wistar rats were administered rAAV1‐IL‐10, rAAV1‐YFP, or saline into the left cerebral artery. Three weeks after gene transfer, rats were subjected to occlusion of the left middle cerebral artery (MCAO) for 45 min followed by reperfusion for 24 h. IL‐10 levels in serum were significantly elevated 3 weeks after rAAV1‐IL‐10 injection, and virus in the cerebral vessels was confirmed by in situ hybridization. Pre‐existing IL‐10 but not YFP decreased the neurological dysfunction scores, brain infarction volume, and the number of injured neuronal cells. AAV1‐IL‐10 transduction increased heme oxygenase (HO‐1) mRNA and protein levels in the infarct boundary zone of the brain. Thus, transduction of the IL‐10 gene in the cerebral artery prior to ischemia attenuates brain injury caused by ischemia/reperfusion in rats. This preventive approach for recurrent stroke can be achieved during interventional treatment for initial stroke. © 2015 IUBMB Life, 67(9):710–719, 2015