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The role of placenta‐derived mesenchymal stem cells in healing of induced full‐thickness skin wound in a mouse model
Author(s) -
AbdAllah Somia H.,
ElShal Amal S.,
Shalaby Sally M.,
AbdElbary Eman,
Mazen Nehad F.,
Abdel Kader Rania R.
Publication year - 2015
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1002/iub.1427
Subject(s) - wound healing , mesenchymal stem cell , vascular endothelial growth factor , proinflammatory cytokine , medicine , placenta , andrology , intraperitoneal injection , reverse transcription polymerase chain reaction , immunology , pathology , vegf receptors , pharmacology , inflammation , cancer research , chemistry , biology , messenger rna , fetus , gene , genetics , pregnancy , biochemistry
We examined the effect of placenta‐derived MSCs (PDMSCs) injection intraregionally and intraperitoneally on healing of induced full thickness mice skin wounds; moreover, the mechanisms by which MSCs exert their effects were also studied. Sixty female mice were divided into three groups after induction of full thickness skin wound; untreated group, wounded mice were injected with MSCs derived from human placenta intraperitoneally or intraregionally. Skin biopsies were obtained 7 and 12 days after wound incision for histological examinations, detection of vascular endothelial growth factor (VEGF) by ELISA, and estimation of expression of mouse ICAM‐1, Integrin β1, Integrin β3 genes and human albumin and GAPDH genes by reverse transcription polymerase chain reaction. Human placenta derived‐MSCs treated groups showed accelerated wound healing than non‐treated group. VEGF, Integrin β1, and Integrin β3 levels were significantly increased in the intraregionally and intraperitoneally treated mice as compared to non‐treated group at day 7 after wound induction. ICAM‐1 showed significant decrease in its expression in treated groups compared with non‐treated group. Interestingly, the intraperitoneal MSCs injections showed better results than intraregional one. PDMSCs accelerate full thickness skin wound healing and the intraperitoneal MSCs injections are more effective than intraregional one. MSCs promote wound healing through release of proangiogenic factors as VEGF, increase healing promoting factors as integrin β1 and β3, and decrease proinflammatory cytokines as ICAM‐1. © 2015 IUBMB Life, 67(9):701–709, 2015