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N ‐Benzylcinnamide induces apoptosis in HPV 16 and HPV 18 cervical cancer cells via suppression of E 6 and E 7 protein expression
Author(s) -
Xiong Yuanhuan,
Chen Lin,
Luo Puying
Publication year - 2015
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1002/iub.1380
Subject(s) - apoptosis , protein expression , microbiology and biotechnology , cancer research , cervical carcinoma , cervical cancer , cancer , expression (computer science) , chemistry , biology , genetics , gene , computer science , biochemistry , programming language
Seventy percent of all cervical cancers are caused by human papillomavirus (HPV) infections. Natural products are being extensively explored for their potential ability to prevent and treat cervical cancers. N ‐benzylcinnamide (PT‐3) is a natural product purified from Piper submultinerve . Whether or not PT‐3 has an effect on cervical cancer cells is as yet unknown. Therefore, we set out to explore the mechanism of action behind PT‐3 and how it affects cells that either contain or lack HPV DNA. Our results demonstrate that PT‐3 slows the growth kinetics of CaSki (HPV‐16 positive) and HeLa (HPV‐18 positive) cells in a dose‐dependent manner, but does not slows HPV‐negative cells. Importantly, we also found that PT‐3 induces apoptosis by suppressing expression of E6 and E7 viral oncogenes in HPV‐infected cervical cancer CaSki and HeLa cells. Moreover, we found that suppression of E6 and E7 expression leads to modulations in p53 and protein retinoblastomas, which are not changed in HPV‐negative cervical cancer C33A cells. These findings demonstrate that PT‐3 can effectively promote apoptosis by downregulating expression of E6 and E7. © 2015 IUBMB Life, 2015 67(5):374–379, 2015