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Pinocembrin inhibits matrix metalloproteinase expression in chondrocytes
Author(s) -
Zhang Dawei,
Huang Bo,
Xiong Chengjie,
Yue Zhou
Publication year - 2015
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1002/iub.1343
Subject(s) - matrix metalloproteinase , chemistry , tumor necrosis factor alpha , iκbα , extracellular matrix , phosphorylation , cartilage , matrix (chemical analysis) , microbiology and biotechnology , nf κb , biochemistry , biology , endocrinology , signal transduction , anatomy , chromatography
Osteoarthritis (OA), the most common form of arthritis, affects millions of people worldwide. The degradation of extracellular matrix induced by matrix metalloproteinases (MMPs) is an important cause of cartilage destruction. Pinocembrin (PB) is one of the primary flavonoids abundant in propolis and extracted as a pure compound. The protective effects of PB in OA have not been reported before. In this study, we found that PB inhibits the expression of MMP‐1, MMP‐3, and MMP‐13 at both mRNA levels and protein levels in human chondrocytes. Importantly, the results of luciferase reporter assay indicated that tumor necrosis factor‐alpha (TNF‐α) induced the activation of nuclear factor kappa‐light‐chain‐enhancer of activated B cells (NF‐κB) was inhibited by the treatment with PB. It is also shown that TNF‐α‐induced p65 nuclear translocation was blocked by the treatment with PB. Mechanistically, PB treatment significantly inhibited TNF‐α‐induced phosphorylation and degradation of the NF‐κB inhibitor IκBα in human chondrocytes. These results suggest a potential protective effect of PB in OA. © 2015 IUBMB Life, 67(1):36–41, 2015