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Effects of sulpiride on mRNA levels of steroid 5α‐reductase isozymes in prostate of adult rats
Author(s) -
Sánchez Pilar,
Torres Jesús M.,
Vílchez Pablo,
del Moral Raimundo G.,
Ortega Esperanza
Publication year - 2008
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1002/iub.13
Subject(s) - sulpiride , testosterone (patch) , prostate , endocrinology , prolactin , medicine , dihydrotestosterone , prostate cancer , androgen , finasteride , chemistry , hormone , cancer , dopamine , dopaminergic
Abstract Prolactin (PRL) is implicated in prostate growth and in the development and regulation of benign prostatic hypertrophy (BPH) and prostate cancer (PCa). PRL may exert its effects on prostate in synergism with androgens. The most active androgen in the prostate is the 5α‐dihydrotestosterone (DHT) obtained from testosterone by the 5α‐reductase (5α‐R) enzyme, which is expressed in the prostate as two isozymes, 5α‐R1 and 5α‐R2. In this study, sulpiride, a prolactin‐secretion inductor, was administered to male rats. mRNA levels of 5α‐R1 and 5α‐R2 were measured in prostate of controls and sulpiride‐treated rats, using one‐step quantitative RT‐PCR coupled with laser‐induced fluorescence capillary electrophoresis (LIF‐CE). Results demonstrated that sulpiride‐induced hyperprolactinemia is associated with an increase in mRNA levels of both 5α‐R1 and 5α‐R2 in prostate of adult rats. Although a direct effect of sulpiride on prostate gland cannot be ruled out, hyperprolactinemia may be a factor to be considered in aging males, in whom prostatic diseases such as BPH and PCa are more frequent. © 2007 IUBMB IUBMB Life, 60(1): 68–72, 2008