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Ambient pH sensing in filamentous fungi: Pitfalls in elucidating regulatory hierarchical signaling networks
Author(s) -
Rossi Antonio,
Cruz Aline H. S.,
Santos Rodrigo S.,
Silva Patrícia M.,
Silva Emiliana M.,
Mendes Niege S.,
MartinezRossi Nilce M.
Publication year - 2013
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1002/iub.1217
Subject(s) - aspergillus nidulans , proteolysis , effector , transcription factor , biochemistry , biology , gene , enzyme , signal transduction , alkaline phosphatase , regulator , metabolic pathway , chemistry , microbiology and biotechnology , mutant
In this article, the experiments used to construct the ambient pH‐signaling network involved in the secretion of enzymes by filamentous fungi have been reviewed, focusing on the phosphate‐repressible phosphatases in Aspergillus nidulans . Classic and molecular genetics have been used to demonstrate that proteolysis of the transcription factor PacC at alkaline ambient pH is imperative for its action, implying that the full‐length version is not an active molecular form of PacC. It has been hypothesized that the transcriptional regulator PacC may be functional at both acidic and alkaline ambient pH, in either the full‐length or the proteolyzed form, if it carries a pal ‐dependent molecular tag. The products of the pal genes are involved in a metabolic pathway that led to the synthesis of effector molecules that tag the pacC product, perhaps facilitating its proteolysis. © 2013 IUBMB Life, 65(11):930–935, 2013