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Inhibition of protein glycation by procyanidin‐B2 enriched fraction of cinnamon: Delay of diabetic cataract in rats
Author(s) -
Muthenna Puppala,
Raghu Ganugula,
Akileshwari Chandrasekhar,
Sinha Sukesh Narayana,
Suryanarayana Palla,
Reddy Geereddy Bhanuprakash
Publication year - 2013
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1002/iub.1214
Subject(s) - proanthocyanidin , glycation , chemistry , fractionation , diabetes mellitus , biochemistry , pharmacology , medicine , endocrinology , antioxidant , chromatography , polyphenol , receptor
Accumulation of advanced glycation endproducts (AGE) from nonenzymatic glycation of proteins has been implicated in several diabetic complications including diabetic cataract. Previously, we have reported that extracts of dietary agents such as cinnamon have the potential to inhibit AGE formation. In this study, we have shown procyanidin‐B2 as the active component of cinnamon that is involved in AGE inhibition using bioassay‐guided fractionation of eye lens proteins under in vitro conditions. The data indicate that procyanidin‐B2 enriched fraction scavenges dicarbonyls. Further, procyanidin‐B2 fraction of cinnamon inhibited the formation of glycosylated hemoglobin in human blood under ex vivo conditions. We have also demonstrated the physiological significance of procyanidin‐B2 fraction in terms of delay of diabetic cataract through inhibition of AGE in diabetic rats. These findings establish the antiglycating potential of procyanidin‐B2 fraction of cinnamon which suggests a scope for controlling AGE‐mediated diabetic complications by food sources that are rich in proanthocyanidins like procyanidin‐B2. © 2013 IUBMB Life, 65(11):941–950, 2013