Premium
Dopaminergic therapies modulate the T‐CELL proteome of patients with Parkinson's disease
Author(s) -
Alberio Tiziana,
Pippione Agnese C.,
Comi Cristoforo,
Olgiati Simone,
Cecconi Daniela,
Zibetti Maurizio,
Lopiano Leonardo,
Fasano Mauro
Publication year - 2012
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1002/iub.1073
Subject(s) - dopaminergic , proteome , dopamine , proteasome , protein subunit , parkinson's disease , biology , medicine , endocrinology , microbiology and biotechnology , biochemistry , disease , gene
Dopamine receptor agonists and L ‐dihydroxyphenylalanine ( L ‐DOPA) counteract dopamine loss in the striatum and are therefore used in the treatment of Parkinson's disease (PD). T‐Lymphocytes express some features of the dopaminergic system, and their function or activation might be regulated by dopaminergic treatments. Two‐dimensional electrophoresis of total protein extract from T‐lymphocytes was performed to identify therapy‐induced proteome changes in T‐cells of 17 patients with PD. Specific protein level alterations were further validated by Western blotting. Of 17 enrolled patients, 11 were treated with different doses of L ‐DOPA; in this group, we found that the levels of two spots, corresponding to ATP synthase subunit β and proteasome subunit β type‐2, correlated linearly with the L‐DOPA daily dose. Moreover, we identified seven proteins (prolidase, actin‐related protein 2, F‐actin‐capping protein subunit β, tropomyosin α‐3 chain, proteasome activator complex subunit 1, peroxiredoxin 6, and a glyceraldehyde‐3‐phosphate dehydrogenase isoform) whose levels were significantly different in patients treated with dopamine agonists. These findings demonstrate that dopaminergic stimulation has important effects on T‐cell proteome in patients under long‐term treatment. Therefore, therapies acting on the dopaminergic system may have additional effects on the immune system. © 2012 IUBMB, IUBMB Life, 64(10):846–852, 2012