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Decreased tumstatin‐mRNA is associated with poor outcome in patients with NSCLC
Author(s) -
Luo YiQin,
Ming Zhou,
Zhao Liang,
Yao LiJuan,
Dong Hang,
Du JianPing,
Wu ShuangZheng,
Hu Wen
Publication year - 2012
Publication title -
iubmb life
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.132
H-Index - 113
eISSN - 1521-6551
pISSN - 1521-6543
DOI - 10.1002/iub.1016
Subject(s) - angiogenesis , messenger rna , immunostaining , cancer research , pathology , immunohistochemistry , biology , medicine , gene , biochemistry
Tumstatin is a candidate tumor suppressor that plays an important role in tumor growth and angiogenesis. The purpose of this study was to evaluate the correlation between tumstatin‐mRNA expression and the clinicopathologic characteristics, tumor angiogenesis, outcome of patients with non–small cell lung cancer (NSCLC). Specimens from 68 patients with NSCLC were recruited in this study. Tumstatin‐mRNA expression and protein level in tumor tissues were quantified respectively by quantitative RT‐PCR and ELISA. Microvessel density (MVD) was determined by CD34 immunostaining. The correlation of tumstatin‐mRNA expression levels with clinicopathologic variables, tumor angiogenesis, and prognosis was analyzed. Tumstatin‐mRNA expression levels were decreased in tumor. Tumstatin‐mRNA expression level was significantly correlated with its protein level in tumor ( r = 0.562; P = 0.001). Tumstatin‐mRNA expression levels in poorly differentiated tumor tissues were significantly lower than in well‐differentiated tumor tissues ( P < 0.004). Furthermore, tumor tumstatin‐mRNA expression were also significantly related to tumor pathologic stage ( P = 0.032) and MVD ( r = −0.77, P < 0.0001). Overall survival (OS) and disease‐free survival (DFS) analysis indicated that NSCLC patients with low tumstatin‐mRNA expression had poorer OS and DFS than those with high expression ( P = 0.015 and 0.037; respectively). Multivariable Cox regression analysis revealed that the tumstatin‐mRNA expression could be an independent prognostic indicators in both DFS and OS. Tumstatin‐mRNA expression levels are down‐regulated in NSCLC tissues. Tumstatin‐mRNA expression level correlates with prognosis, which suggests that tumstatin‐mRNA is a new potential independent marker of favorable prognosis in NSCLC. 2012 IUBMB IUBMB Life, 2012