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ATP and Ergosterol as Indicators of Fungal Biomass during Leaf Decomposition in Streams: a Comparative Study
Author(s) -
Abelho Manuela
Publication year - 2009
Publication title -
international review of hydrobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.524
H-Index - 52
eISSN - 1522-2632
pISSN - 1434-2944
DOI - 10.1002/iroh.200811093
Subject(s) - ergosterol , biomass (ecology) , respiration , spore , decomposition , hyphomycetes , botany , zoology , chemistry , biology , dry weight , food science , environmental chemistry , ecology
Ergosterol and ATP concentrations, microbial respiration and sporulation rates of aquatic hyphomycetes associated with leaves of Castanea sativa decomposing in a 5 th order stream were determined periodically over a period of 102 days in order to compare ergosterol and ATP as indicators of fungal biomass. ATP and ergosterol concentrations exhibited a significant positive correlation ( F = 4.459, DF = 28, P < 0.001) during the first stages of leaf breakdown (until day 39), i.e. , during periods of increasing fungal biomass. No correlation was found between ATP and ergosterol concentrations during later stages of decomposition (days 39 to 102). Respiration rates increased rapidly up to 0.525 mg O 2 h – 1 g – 1 AFDM during the first month and remained high until the end of the experiment. Sporulation rates peaked at day 9 (1069 conidia day – 1 mg – 1 AFDM) and decreased during later stages of decomposition. ATP‐to‐biomass conversion factors were determined for both fungi (0.59 μmol ATP g – 1 dry mass) and bacteria (1.30 μmol ATP g – 1 dry mass) collected from the stream and grown in the laboratory. Estimates of fungal biomass based on ATP concentrations were similar to those calculated from ergosterol concentrations during the first 39 days of breakdown. The results here presented suggest that ATP is a reliable method to quantify microbial biomass in streams and that the relative importance of bacteria increases at later stages of decomposition. (© 2009 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)