A Method Based on Artificial Substrates to Monitor Hyporheic Biofilm Development | Zendy

Claret Cecile | Zendy

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A Method Based on Artificial Substrates to Monitor Hyporheic Biofilm Development

Author(s) -

Claret Cecile

Publication year - 1998

Publication title -

international review of hydrobiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.524

H-Index - 52

eISSN - 1522-2632

pISSN - 1434-2944

DOI - 10.1002/iroh.19980830204

Subject(s) - biofilm , riffle , sonication , hyporheic zone , bacteria , environmental science , colonization , substrate (aquarium) , chemistry , ecology , biology , environmental engineering , surface water , chromatography , genetics , habitat

A method based on artificial substrata is proposed to study hyporheic biofilm development as well as the procedure to remove bacteria from these biofilms. The device is a perforated pipe in which glass slides are introduced vertically. Attached bacteria are removed by sonication for 180 seconds at power 2 (arbitrary unit); longer sonication time or higher power can cause cell destruction. A bacterial colonization experiment was performed in an upwelling zone of a riffle in the Rhǒne River. Total bacterial abundances reached a steady‐state after 5 days of colonization, and the percentage of ETS‐active bacteria was maximal after 3 days of incubation in the bed sediments. These results validate this device as usable to monitor biofilm development in natural systems, making this method as a promising tool to characterize the trophic status of a stream using integrative biological descriptors.

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