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Application of testicular spermatozoa cryopreservation in assisted reproduction
Author(s) -
Yu Guanling,
Liu Yujin,
Zhang Haozhen,
Wu Keliang
Publication year - 2018
Publication title -
international journal of gynecology and obstetrics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.895
H-Index - 97
eISSN - 1879-3479
pISSN - 0020-7292
DOI - 10.1002/ijgo.12537
Subject(s) - intracytoplasmic sperm injection , medicine , cryopreservation , andrology , gynecology , blastocyst , human fertilization , sperm , infertility , pregnancy rate , live birth , pregnancy , male infertility , embryo , biology , embryogenesis , anatomy , genetics , microbiology and biotechnology
Objective To evaluate the efficiency of spermatozoa cryopreservation and to compare the clinical pregnancy outcomes in intracytoplasmic sperm injection ( ICSI ) using fresh versus cryopreserved spermatozoa collected by testicular sperm aspiration ( TESA ). Methods A retrospective study was performed to compare the outcomes of men who accepted frozen‐spermatozoa‐based TESA ‐ ICSI with those of men who underwent TESA ‐ ICSI using fresh spermatozoa between January 1, 2015, and December 30, 2016. The groups were matched for age. The rates of fertilization, good‐quality embryos, blastocyst formation, and clinical pregnancy outcomes were obtained from clinical records and were compared between the groups. Results There were no significant differences between the frozen TESA group (n=79) and the fresh TESA group (n=194) in the rates of fertilization (71.4% vs 73.4%), good‐quality embryos (55.3% vs 54.5%), blastocyst formation (60.9% vs 60.1%), clinical pregnancy (61.7% vs 55.1%), and live delivery (51.1% vs 45.7%) ( P >0.05 for all comparisons). Conclusion Freezing low‐count sperm collected by TESA with a cryoprotectant was an efficient method in the treatment of male factor infertility.