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Associations between intratumoral and peritumoral M2 macrophage counts and cervical squamous cell carcinoma invasion patterns
Author(s) -
Li Yuan,
Huang Gaosheng,
Zhang Shaohua
Publication year - 2017
Publication title -
international journal of gynecology and obstetrics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.895
H-Index - 97
eISSN - 1879-3479
pISSN - 0020-7292
DOI - 10.1002/ijgo.12320
Subject(s) - medicine , tissue microarray , cd163 , pathology , immunohistochemistry , cervical cancer , ki 67 , carcinoma , basal cell , infiltration (hvac) , cancer , macrophage , biology , biochemistry , in vitro , physics , thermodynamics
Objective To study the correlation between M2 tumor‐associated macrophages ( TAM s) and invasion patterns in cervical squamous cell carcinoma ( SCC ) tissues. Methods In the present observational study, two commercial SCC tissue microarrays were evaluated by immunohistochemical analysis between January 1, 2016, and December 31, 2016. CD 163‐positive cells in a cervical SCC tissue microarray were identified. Tissue samples were stratified into groups based on a pushing border pattern ( PBP ), a diffuse infiltration pattern ( DIP ), or non‐tumorous tissues included as a control group; M2 TAM numbers and distributions were compared. Results The microarrays included 109 cervical SCC samples and 45 non‐tumorous control samples. The mean number of intratumoral M2 TAM s in cervical SCC tissue samples (35.8 ± 28.3) was significantly higher than the number of intraepithelial M2 TAM s in non‐tumorous cervical samples (1.2 ± 3.6) ( P< 0.001); similarly, the number of peritumoral M2 TAM s in cervical SCC (44.7 ± 29.4) was higher than in non‐tumorous cervical tissues (10.4 ± 9.2) ( P< 0.001). The numbers of intratumoral ( P= 0.046) and peritumoral ( P= 0.002) M2 TAM s were higher among the DIP group compared with the PBP group. Conclusion A close relationship was identified between M2 TAM s and invasion patterns in cervical SCC . Further, M2 TAM infiltration was more pronounced in DIP compared with PBP SCC tissue samples.