Premium
1 H‐decoupling and Isotopic Labeling for the Measurement of the Longitudinal Relaxation Time of Hyperpolarized 13 C‐Methylenes in Choline Analogs
Author(s) -
Uppala Sivaranjan,
Gamliel Ayelet,
Harris Talia,
Sosna Jacob,
Gomori J. Moshe,
Jerschow Alexej,
KatzBrull Rachel
Publication year - 2019
Publication title -
israel journal of chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.908
H-Index - 54
eISSN - 1869-5868
pISSN - 0021-2148
DOI - 10.1002/ijch.201900016
Subject(s) - chemistry , hyperpolarization (physics) , decoupling (probability) , deuterium , analytical chemistry (journal) , polarization (electrochemistry) , nuclear magnetic resonance , nuclear magnetic resonance spectroscopy , atomic physics , stereochemistry , physics , chromatography , control engineering , engineering
The T 1 of a hyperpolarized site in solution is a key parameter that determines the time‐window in which its NMR signals are observable. For 13 C sites adjacent to protons, 1 H‐decoupling has been shown to increase the hyperpolarized signal resolution and SNR. Additionally, polarization transfer to protons has shown utility in increasing the sensitivity of detection. However, 1 H‐decoupling could lead to a change in the decay rate of a hyperpolarized 13 C site. Here we tested this possible effect in a case where the protons are directly bound to an sp 3 hyperpolarized 13 C site (using [1,2‐ 13 C 2 ]choline) and 1 H‐decoupling was applied continuously throughout the hyperpolarized decay measurement. We found that 1 H‐decoupling did not lead to any significant changes in the 13 C polarization decay time but did result in the expected collapse of J‐coupling and produced sharper signals. This result suggested that 1 H‐decoupling did not affect the decay rate of hyperpolarized sp 3 13 C sites. The deuterium‐substitution approach (using [1,1,2,2‐D 4 ,1‐ 13 C]choline) showed a dramatic prolongation of T 1 . Upper bounds on the T 1 of all investigated sites were calculated.