Guest‐responsive, Non‐proteolytic Harvest of a Cell‐sheet using Controllable Host‐guest Chemistry | Zendy

Park Kyeng Min | Zendy; Shrinidhi Annadka | Zendy; Murray James | Zendy; Kim Kimoon | Zendy

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Guest‐responsive, Non‐proteolytic Harvest of a Cell‐sheet using Controllable Host‐guest Chemistry

Author(s) -

Park Kyeng Min,

Shrinidhi Annadka,

Murray James,

Kim Kimoon

Publication year - 2018

Publication title -

israel journal of chemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.908

H-Index - 54

eISSN - 1869-5868

pISSN - 0021-2148

DOI - 10.1002/ijch.201700084

Subject(s) - chemistry , hyaluronic acid , host (biology) , conjugated system , dermal fibroblast , polymer , biophysics , fibroblast , polymer chemistry , nanotechnology , biochemistry , organic chemistry , anatomy , in vitro , medicine , ecology , biology , materials science

A new non‐proteolytic method to harvest a cell‐sheet was demonstrated using controllable host‐guest interactions which can be dissociated by treating a strong guest on demand. Fibroblast cells (NIH3T3) were grown to confluence on a 1,6‐diammoniumhexane conjugated hyaluronic acid (DAH‐HA) polymer which was anchored to a cucurbit[7]uril (CB[7]) surface using the host‐guest interaction between DAH and CB[7]. Treating with a strong guest allowed the cultured cells to be detached from the surface as a free standing sheet. This approach demonstrated the great potential of controllable host‐guest chemistry as a novel tool for non‐proteolytic harvesting of cell‐sheets useful for regenerative therapy.

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