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wPMLG‐5 Spectroscopy of Self‐Aggregated BChl e in Natural Chlorosomes of Chlorobaculum Limnaeum
Author(s) -
Miloslavina Yuliya,
Gupta Karthick Babu Sai Sankar,
Tank Marcus,
Bryant Donald A.,
de Groot Huub J. M.
Publication year - 2014
Publication title -
israel journal of chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.908
H-Index - 54
eISSN - 1869-5868
pISSN - 0021-2148
DOI - 10.1002/ijch.201300129
Subject(s) - chlorosome , chemistry , spectroscopy , nuclear magnetic resonance spectroscopy , bacteriochlorophyll , magic angle spinning , solid state nuclear magnetic resonance , molecule , chemical physics , two dimensional nuclear magnetic resonance spectroscopy , nuclear magnetic resonance , physics , stereochemistry , quantum mechanics , organic chemistry , pigment
1 H magic angle spinning (MAS) nuclear magnetic resonance (NMR), employing rapid spinning and the wPMLG‐5 pulse sequence, was used to explore the possibility for performing 1 H solid state NMR of a light‐harvesting organelle, the chlorosome antenna of Chlorobaculum limnaeum . This natural antenna system is built from bacteriochlorophyll e (BChl e ) molecules that are self‐assembled to form a supramolecular scaffold for in vivo harvesting of light. We present preliminary data on this chlorosome species and address the feasibility of performing wPMLG spectroscopy, in terms of high power irradiation on a fragile biological sample. In parallel, enhancing the 1 H shift dispersion from the magnetic field can help to resolve signals from protons that resonate downfield. Different line narrowing mechanisms operating in parallel provide access to resolving selected 1 H signals collected from the moderately sized and chemically diverse BChl e molecular motif in the chlorosome scaffold. These discoveries will be helpful for future studies of structural and functional characteristics of self‐assembled natural and artificial light‐harvesting molecules.