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Photoactivation and Detection of Photoexcited Molecules and Photochemical Products
Author(s) -
Kozinska Anna,
Oles Tomasz,
Sarna Tadeusz
Publication year - 2012
Publication title -
israel journal of chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.908
H-Index - 54
eISSN - 1869-5868
pISSN - 0021-2148
DOI - 10.1002/ijch.201200019
Subject(s) - chemistry , singlet oxygen , photochemistry , reactive oxygen species , radical , molecule , photodynamic therapy , reactivity (psychology) , excited state , oxygen , substrate (aquarium) , reactive intermediate , organic chemistry , catalysis , biochemistry , medicine , physics , alternative medicine , oceanography , pathology , nuclear physics , geology
Aerobic photoactivation of photosensitizing dye molecules can lead to the formation of oxygen radicals, singlet oxygen and other partially reduced oxygen species, collectively called reactive oxygen species (ROS), which are responsible for photodynamic damage and the accompanying cytotoxicity. This review briefly describes basic photophysical phenomena involved in the formation of electronically excited states and photochemical processes that play a key role in the generation of ROS. Physicochemical properties of the excited states of the photosensitizing dye molecules and of ROS, particularly their chemical reactivity with selected substrate molecules, as well as major spectroscopic and analytical methods used for the detection and characterization of reactive intermediates involved in photodynamic phenomena, are critically discussed in this paper.