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Soft Immobilization of Proteins onto Single‐Walled Carbon Nanotubes through Nickel Complexed Nitrilotriacetic Acid‐End Functionalized Polystyrenes
Author(s) -
Kadir Mohammad Abdul,
Park Jinhong,
Kim BongSoo,
Lee SunGu,
Paik Hyunjong
Publication year - 2012
Publication title -
israel journal of chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.908
H-Index - 54
eISSN - 1869-5868
pISSN - 0021-2148
DOI - 10.1002/ijch.201100119
Subject(s) - nitrilotriacetic acid , chemistry , carbon nanotube , atom transfer radical polymerization , imidazole , polystyrene , nickel , polymer chemistry , histidine , polymer , green fluorescent protein , polymerization , nuclear chemistry , chemical engineering , inorganic chemistry , organic chemistry , chelation , enzyme , biochemistry , engineering , gene
We describe the immobilization of histidine‐tagged green fluorescent protein (His 6 ‐GFP) specifically and reversibly onto single‐walled carbon nanotubes (SWCNTs) using nickel complexed nitrilotriacetic acid‐end functionalized polystyrene (Ni‐NTA‐PS) by conjugation between the protein and the polymer in phosphate buffer solution (PBS). We synthesized Ni‐NTA‐PS by atom transfer radical polymerization (ATRP) using a nitrilotriacetic acid (NTA) initiator. The SWCNTs were dispersed in PBS along with Ni‐NTA‐PS and His 6 ‐GFP aided by sonication. The diameter of the dispersed SWCNTs was measured by transmission electron microscope (TEM). The immobilization of His 6 ‐GFP and dispersion of SWCNTs were controlled by addition of excess imidazole. It was found that the immobilized His 6 ‐GFP was more stable than free His 6 ‐GFP in organic solvent (PBS/DMF, 1/1, v/v).