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Selenoglutathione‐Mediated Rescue of Kinetically Trapped Intermediates in Oxidative Protein Folding
Author(s) -
Metanis Norman,
Foletti Carlotta,
Beld Joris,
Hilvert Donald
Publication year - 2011
Publication title -
israel journal of chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.908
H-Index - 54
eISSN - 1869-5868
pISSN - 0021-2148
DOI - 10.1002/ijch.201100105
Subject(s) - chemistry , oxidative folding , folding (dsp implementation) , oxidative phosphorylation , reagent , protein folding , catalysis , diselenide , biophysics , photochemistry , cysteine , biochemistry , organic chemistry , selenium , enzyme , electrical engineering , biology , engineering
Selenoglutathione has been shown to have considerable potential as a catalyst of oxidative protein folding. Here we examine how this reagent modulates the folding pathway of bovine pancreatic trypsin inhibitor (BPTI) and show that the diselenide increases the efficiency of this process primarily by accelerating the conversion of a kinetically trapped folding intermediate.