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Chemical Synthesis of Integral Membrane Proteins: Methods and Applications
Author(s) -
Shen Fei,
Huang YiChao,
Tang Shan,
Chen YongXiang,
Liu Lei
Publication year - 2011
Publication title -
israel journal of chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.908
H-Index - 54
eISSN - 1869-5868
pISSN - 0021-2148
DOI - 10.1002/ijch.201100076
Subject(s) - chemistry , integral membrane protein , semisynthesis , membrane protein , transmembrane protein , membrane , peptide , peptide synthesis , chemical synthesis , biochemistry , combinatorial chemistry , in vitro , receptor
The development of efficient chemical methods for total synthesis or semisynthesis of integral membrane proteins is an important challenge at the interface between chemistry and biology. This review outlines the recent advances in the synthesis of integral membrane proteins, with particular focus on the methods for difficult peptide synthesis, purification, and enhancement of peptide solubility under the ligation conditions. The applications of these methods to the synthesis of integral membrane proteins with one or multiple transmembrane domains are also described.