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RNA Interference‐Based Computing in Mammalian Cells
Author(s) -
Benenson Yaakov
Publication year - 2011
Publication title -
israel journal of chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.908
H-Index - 54
eISSN - 1869-5868
pISSN - 0021-2148
DOI - 10.1002/ijch.201000070
Subject(s) - rna interference , interference (communication) , scalability , synthetic biology , chemistry , computational biology , rna , exploit , transcription (linguistics) , logic gate , microbiology and biotechnology , nanotechnology , computer science , gene , biology , biochemistry , telecommunications , algorithm , channel (broadcasting) , computer security , philosophy , materials science , database , linguistics
Development of synthetic biological pathways is contingent upon the availability of general‐purpose, programmable tools to process molecule‐encoded information in live cells. A few years ago we introduced a comprehensive design framework for biological computing in mammalian cells that utilizes the RNA interference (RNAi) pathway. Our method exploits the relatively simple structural requirements of the RNAi to engineer increasingly complex molecular logic circuits. This led to a scalable and intuitive method to perform logic integration of transcription factors, and first steps toward sensing and integrating mRNA signals.