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Chemically‐Specific Probes for the Atomic Force Microscope
Author(s) -
Lee Gil U,
Chrisey Linda A.,
O'Ferrall C. Elizabeth,
Pilloff Daniel E.,
Turner Noel H.,
Colton Richard J.
Publication year - 1996
Publication title -
israel journal of chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.908
H-Index - 54
eISSN - 1869-5868
pISSN - 0021-2148
DOI - 10.1002/ijch.199600011
Subject(s) - chemistry , atomic force microscopy , nanotechnology , nanometre , molecule , covalent bond , oligonucleotide , macromolecule , resolution (logic) , microscope , chemical engineering , organic chemistry , optics , dna , biochemistry , materials science , physics , artificial intelligence , computer science , engineering
The atomic force microscope (AFM) measures force and displacement with high sensitivity and submillisecond temporal resolution. By functionalizing the AFM probe with specific chemical groups or macromolecules it is possible to characterize the chemical and physical properties of single molecules on the nanometer scale. In this paper we discuss the key issues that must be addressed when designing and characterizing a successful immobilization chemistry, and describe the chemistry we developed to covalently immobilize oligonucleotides in a specific orientation.