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H 2 O 2 ‐Diffusion Through Liposomes
Author(s) -
Frimer Aryeh A.,
Forman Arthur,
Borg Donald C.
Publication year - 1983
Publication title -
israel journal of chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.908
H-Index - 54
eISSN - 1869-5868
pISSN - 0021-2148
DOI - 10.1002/ijch.198300064
Subject(s) - chemistry , liposome , chromogenic , horseradish peroxidase , bilayer , peroxidase , membrane , lipid bilayer , diffusion , phenol , biophysics , chromatography , biochemistry , enzyme , organic chemistry , physics , biology , thermodynamics
The toxicity of H 2 O 2 suggests that it readily diffuses through cell membranes. The present paper demonstrates via a chromogenic peroxidase reaction that H 2 O 2 can cross the lipid bilayer membrane without affecting the cell wall's integrity. In this demonstration, the chromogen ABTS was encapsulated with horseradish peroxidase into ovolecithin multilamellar liposomes. Upon the addition of dilute H 2 O 2 , the color was generated exclusively within the intact liposomes. Related results were observed with the AAP/DHBS chromogenic couple; however, this system is complicated by the small holes created by the phenol DHBS in the liposomal wall.