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Photosensitized Dimerization of Thymine and Cytosine in DNA
Author(s) -
BenIshai R.,
BenHur E.,
Hornfeld Y.
Publication year - 1968
Publication title -
israel journal of chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.908
H-Index - 54
eISSN - 1869-5868
pISSN - 0021-2148
DOI - 10.1002/ijch.196800094
Subject(s) - chemistry , thymine , cytosine , pyrimidine dimer , dna , photochemistry , acetophenone , dimer , stereochemistry , acetone , photosensitizer , biochemistry , dna damage , organic chemistry , catalysis
Abstract Thymine and cytosine homo‐ and heterodimers ate produced when E. coli DNA is irradiated at wavelength > 300 mμ in the presence of acetone as photosensitizer. Employing acetophenone as sensitizer only thymine containing dimers are obtained. In heat denatured DNA photosensitized dimerization of thymine occurs in a higher proportion of the TT dinucleotide sequences than in native DNA and the trans‐syn thymine dimer isomer is formed as a minor photoproduct.