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Structural Factors in Respiratory Stimulation by Na + ‐ions in Escherichia Coli
Author(s) -
Kahana Luna,
AviDor Y.
Publication year - 1966
Publication title -
israel journal of chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.908
H-Index - 54
eISSN - 1869-5868
pISSN - 0021-2148
DOI - 10.1002/ijch.196600012
Subject(s) - chemistry , escherichia coli , substrate (aquarium) , stimulation , glutamate receptor , respiration , biophysics , ion , biochemistry , organic chemistry , medicine , biology , oceanography , receptor , gene , geology , botany
While K + has a general stimulatory effect on respiration in Escherichia coli, Na + specifically enhances the rate of glutamate oxidation. Na + reduces the apparent K m of the glutamate‐oxidase system with respect to the substrate 90 times, but has no influence on V m. It is only effective when the cell structure remains intact, glutamate concentration is relatively low and hypotonic conditions prevail. In a cell‐free system the kinetic constants are not affected by Na + . Some implications of the above findings are discussed.