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miR ‐17‐3p promotes the proliferation of multiple myeloma cells by downregulating P21 expression through LMLN inhibition
Author(s) -
Xiang Pu,
Yeung Yiu To,
Wang Jiheng,
Wu Qiong,
Du Ruijuan,
Huang Chuntian,
Jia Xuechao,
Gao Yunfeng,
Zhi Yafei,
Guo Fangqin,
Wei Huifang,
Zhang Dandan,
Liu Yuzhang,
Liu Lina,
Liang Lijie,
Wang Juan,
Song Yongping,
Liu Kangdong,
Fang Baijun
Publication year - 2021
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.33528
Subject(s) - monoclonal gammopathy of undetermined significance , multiple myeloma , peripheral blood mononuclear cell , microrna , cell growth , bone marrow , cancer research , plasma cell , apoptosis , malignancy , biomarker , in vivo , cell cycle , cell , medicine , ex vivo , pathology , biology , immunology , monoclonal antibody , cancer , monoclonal , antibody , in vitro , gene , biochemistry , genetics , microbiology and biotechnology
Multiple myeloma (MM), a hematological malignancy, has a poor prognosis and requires an invasive procedure. Reports have implicated miRNAs in the diagnosis, treatment and prognosis of hematological malignancies. In our study, we evaluated the expression profiles of miR‐17‐3p in plasma and bone marrow mononuclear cells of monoclonal gammopathy of undetermined significance (MGUS) and MM patients and healthy subjects. The results showed that the plasma and mononuclear cell expression levels of miR‐17‐3p in MM patients were higher than those in MGUS patients and normal controls. In addition, the expression of miR‐17‐3p was positively correlated with diagnostic indexes, such as marrow plasma cell abundance and serum M protein level, and positively correlated with the International Staging System stage of the disease. Receiver operating characteristic curve analysis suggested that miR‐17‐3p might be a diagnostic index of MM. Moreover, miR‐17‐3p regulated cell proliferation, apoptosis and the cell cycle through P21 in MM cell lines and promoted MM tumor growth in vivo. Furthermore, we predicted and verified LMLN as a functional downstream target gene of miR‐17‐3p. Negatively regulated by miR‐17‐3p, LMLN inhibits MM cell growth, exerting a tumor suppressive function through P21. Taken together, our data identify miR‐17‐3p as a promising diagnostic biomarker for MM in the clinic and unveil a new miR‐17‐3p‐LMLN‐P21 axis in MM progression.