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Intravital imaging of vascular anomalies and extracellular matrix remodeling in orthotopic pancreatic tumors
Author(s) -
Bochner Filip,
Mohan Vishnu,
Zinger Assaf,
Golani Ofra,
Schroeder Avi,
Sagi Irit,
Neeman Michal
Publication year - 2019
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.32759
Subject(s) - pathology , angiogenesis , tumor microenvironment , pancreatic cancer , extracellular matrix , pancreas , tumor progression , pancreatic tumor , medicine , cancer research , biology , cancer , microbiology and biotechnology
Pancreatic cancers, both adenocarcinomas and endocrine tumors are characterized by varying levels of aberrant angiogenesis and fibrotic microenvironment. The difficulty to deliver drugs and treat the disease has been attributed in part to the vascular architecture and tissue/ECM density. Here we present longitudinal three‐dimensional intravital imaging of vascular and tumor microenvironment remodeling in spontaneous transgenic tumors (RIP1‐Tag2 insulinomas) and orthotopically injected tumors (KPC adenocarcinomas). Analysis of the data acquired in insulinomas revealed major differences in tumor blood vessel branching, fraction volume, number of branch points segments, vessel straightness and length compared to the normal tissue. The aggressive adenocarcinoma presented widespread peritumoral vascular remodeling and heterogeneous vascular distribution. Longitudinal imaging was used to acquire sequential vascular remodeling data during tumor progression. This work demonstrates the potential for using a pancreatic intravital imaging window for direct visualization of the tumor heterogenic microenvironments during tumor progression.