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Targeting ferroptosis in rhabdomyosarcoma cells
Author(s) -
Dächert Jasmin,
Ehrenfeld Vanessa,
Habermann Karoline,
Dolgikh Nadezda,
Fulda Simone
Publication year - 2019
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.32496
Subject(s) - programmed cell death , reactive oxygen species , glutathione , lipid peroxidation , oxidative stress , microbiology and biotechnology , chemistry , protein kinase c , biology , biochemistry , apoptosis , kinase , enzyme
Recent data suggest that rhabdomyosarcoma (RMS) cells might be vulnerable to oxidative stress‐induced cell death. Here, we show that RMS are susceptible to cell death induced by Erastin, an inhibitor of the glutamate/cystine antiporter x c − that can increase reactive oxygen species (ROS) production via glutathione (GSH) depletion. Prior to cell death, Erastin caused GSH depletion, ROS production and lipid peroxidation. Importantly, pharmacological inhibitors of lipid peroxidation (i.e., Ferrostatin‐1, Liproxstatin‐1), ROS scavengers (i.e., α‐Tocopherol, GSH) and the iron chelator Deferoxamine inhibited ROS accumulation, lipid peroxidation and cell death, consistent with ferroptosis. Interestingly, the broad‐spectrum protein kinase C (PKC) inhibitor Bisindolylmaleimide I as well as the PKCα‐ and β‐selective inhibitor Gö6976 significantly reduced Erastin‐induced cell death. Similarly, genetic knockdown of PKCα significantly protected RMS cells from Erastin‐induced cell death. Furthermore, the broad‐spectrum nicotinamide adenine dinucleotide phosphate‐oxidase (NOX) inhibitor Diphenyleneiodonium and the selective NOX1/4 isoform inhibitor GKT137831 significantly decreased Erastin‐stimulated ROS, lipid ROS and cell death. These data provide new insights into the molecular mechanisms of ferroptosis in RMS, contributing to the development of new redox‐based treatment strategies.