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Circular RNA CEP128 promotes bladder cancer progression by regulating Mir‐145‐5p/ Myd88 via MAPK signaling pathway
Author(s) -
Sun Ming,
Zhao Wenyan,
Chen Zhaofu,
Li Ming,
Li Shuqiang,
Wu Bin,
Bu Renge
Publication year - 2019
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.32311
Subject(s) - gene knockdown , bladder cancer , cancer research , microrna , biology , cell cycle , mapk/erk pathway , cell growth , viability assay , cell cycle checkpoint , signal transduction , cancer , microbiology and biotechnology , apoptosis , gene , genetics
The present experiment was designed for exploring the regulatory mechanism of circ‐CEP128/miR‐145‐5p/ MYD88 axis in bladder cancer. MiRNAs and circRNAs expression data were derived from Gene Expression Omnibus database with bladder tumor tissues and paracarcinoma tissue samples. Differentially expressed genes in tumor were analyzed via R software. Interaction network of differently expressed miRNAs and differently expressed mRNA was established by means of Cytoscape software. CircCEP128 and miR‐145‐5p expression levels were determined using qRT‐PCR. The expression of MAPK signaling‐related proteins MYD88 , p38, ERK and JNK was examined by western blot. The relationship between circCEP128 and miR‐145‐5p was validated using RNA immunoprecipitation. The level of cell propagation and migration was determined by CCK8 and wound healing assay, 5‐bromo‐2′‐deoxyuridine assay and migration assay. Cell apoptosis rate and cell cycle were detected via flow cytometry. Tumor xenograft assay was implemented to investigate the function of circCEP128 in vivo . CircCEP128 and MYD88 were overexpressed in bladder cancer based on microarray analysis and miR‐145‐5p was a potential targeting factor in bladder cancer. CircCEP128 targeted miR‐145‐5p and miR‐145‐5p targeted MYD88 . Expression of miR‐145‐5p was decreased in cancer samples. Knockdown of circCEP128 induced the inhibition of cell viability and mobility and cell cycle arrest. Overexpression of miR‐145‐5p or knockdown of circCEP128 promoted MAKP signaling pathway and related proteins expression. In addition, knockdown of circCEP128 suppressed the growth of bladder cancer tumor tissues in vivo . Overexpression of circCEP128 promoted bladder cancer progression through modulating miR‐145‐5p and MYD88 via MAKP signaling pathway.