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Long non‐coding RNA NEAT1 is a transcriptional target of p53 and modulates p53‐induced transactivation and tumor‐suppressor function
Author(s) -
Idogawa Masashi,
Ohashi Tomoko,
Sasaki Yasushi,
Nakase Hiroshi,
Tokino Takashi
Publication year - 2017
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.30689
Subject(s) - transactivation , biology , chromatin immunoprecipitation , gene knockdown , suppressor , long non coding rna , cancer research , gene , tumor suppressor gene , transcription (linguistics) , transcriptional regulation , gene expression , microbiology and biotechnology , rna , genetics , carcinogenesis , promoter , linguistics , philosophy
p53 is one of the most important tumor suppressor genes, and the direct transcriptional targets of p53 must be explored to elucidate its functional mechanisms. Thus far, the p53 targets that have been primarily studied are protein‐coding genes. Our previous study revealed that several long non‐coding RNAs (lncRNAs) are direct transcriptional targets of p53, and knockdown of specific lncRNAs modulates p53‐induced apoptosis. In this study, analysis of next‐generation chromatin immunoprecipitation‐sequencing (ChIP‐seq) data for p53 revealed that the lncRNA NEAT1 is a direct transcriptional target of p53. The suppression of NEAT1 induction by p53 attenuates the inhibitory effect of p53 on cancer cell growth and also modulates gene transactivation, including that of many lncRNAs. Furthermore, low expression of NEAT1 is related to poor prognosis in several cancers. These results indicate that the induction of NEAT1 expression contributes to the tumor‐suppressor function of p53 and suggest that p53 and NEAT1 constitute a transcriptional network contributing to various biological functions and tumor suppression.