Premium
Epigenetic suppression of the immunoregulator MZB1 is associated with the malignant phenotype of gastric cancer
Author(s) -
Kanda Mitsuro,
Tanaka Chie,
Kobayashi Daisuke,
Tanaka Haruyoshi,
Shimizu Dai,
Shibata Masahiro,
Takami Hideki,
Hayashi Masamichi,
Iwata Naoki,
Niwa Yukiko,
Yamada Suguru,
Fujii Tsutomu,
Nakayama Goro,
Fujiwara Michitaka,
Kodera Yasuhiro
Publication year - 2016
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.30286
Subject(s) - biology , cancer research , cancer , dna methylation , epigenetics , gene knockdown , transcriptome , tumor progression , immune system , gene expression , cell culture , microbiology and biotechnology , immunology , gene , biochemistry , genetics
Prediction of tumor recurrence after curative resection is critical for determining the prognosis of patients with gastric cancer (GC). The initiation and progression of GC are associated with inappropriate immune responses caused by chronic inflammation of the gastric mucosa. To identify immunoregulatory molecules involved in GC progression, GC cell lines and 200 pairs of tumor and normal tissues from patients with GC were analyzed for gene expression, amplification and methylation as well as function of a differentially expressed gene. The transcriptome analysis revealed that marginal zone B and B1 cell specific protein ( MZB1 ) was expressed at significantly decreased levels in primary GC tissues when compared with the corresponding normal gastric mucosa. PCR array analysis exploring genes expressed cooperatively with MZB1 revealed that differential expression of MZB1 mRNA in GC cell lines correlated positively with the levels of the mRNAs encoding estrogen receptor 1 and desumoylating isopeptidase 1. Hypermethylation of the MZB1 promoter was frequent in cell lines with decreased levels of MZB1 mRNA. siRNA‐mediated knockdown of MZB1 significantly increased proliferation, invasion and migration of GC cell lines. Low MZB1 expression was an independent prognostic factor for recurrence after curative gastrectomy and was associated significantly with increased hematogenous recurrence. MZB1 acts as a suppressor of GC. Low MZB1 expression in the primary GC tissue is predictive of recurrence after curative resection.